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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in many tumors, which results in chromosome instability, and therefore may well add to tumorigenesis. This protein was uncovered to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a critical role in advertising mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, happen to be explained. Two more splice variants ended up documented but comprehensive nucleotide sequence information isn't nevertheless obtainable.
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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide typically has the epitope identified from the antibody.
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This manual outlines even further feasible optimization of cellular and immunoassay parameters to ensure the absolute best success are...
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This guideline outlines additional achievable optimization of mobile and immunoassay parameters to guarantee the best possible effects are...
Antibodies bound to the blocking peptide not bind for the epitope to the concentrate on protein. By comparing the staining from your blocked antibody vs . the antibody by yourself, one can see which staining is precise.
Concentrate on Facts Cyclin E belongs into the highly conserved cyclin household, whose users are characterised by a dramatic periodicity in protein abundance from the mobile cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation patterns which add into the temporal coordination of each and every mitotic celebration. Cyclin E kinds a fancy with and capabilities to be a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S changeover. This protein accumulates within the G1-S phase boundary and is also degraded as cells development as a result of S stage.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...
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